SPIT SEQ- Whole Genome Sequencing of MTB | Micra | MedGenome | MedGenome - Part 18001033691

What is SPIT SEQ?

SPIT SEQ is a direct sputum based whole genome sequencing test for diagnosis and drug resistance testing in pulmonary tuberculosis

Prevalence

  • Worldwide, tuberculosis (TB) is one of the top 10 causes of death, and the leading cause from a single infectious agent (above HIV/AIDS). According to a WHO report, India saw 2.7 million TB cases (incidence + relapse) in 2017. India accounted for 27% of global TB deaths. Globally, 3.4% of new TB cases and 18% of previously treated cases had multi- drug resistant/rifampicin resistant TB (MDR/RR-TB). India is one of the top 3 countries with the largest number of MDR/RR-TB cases that constitute 43% of global MDR/RR-TB cases

Common Disorders

SPIT SEQ, MedGenome’s Mtb Test sequences the whole genome of Mycobacterium Tuberculosis (Mtb) from the sputum of patients suffering from tuberculosis.

Why do you need the test?

  • Single test for diagnosis and drug resistance prediction – As the test includes sequencing the Mtb genome, it can diagnose the presence of Mtb as well as identify the mutations that are likely to cause drug resistance.
  • Comprehensive Drug Panel – The existing molecular techniques have a limitation of covering only a few drugs. The whole genome sequencing of Mtb covers all drug resistance markers – reported as well as novel.
  • Sample Requirement – The SPIT SEQ test uses direct sputum as the clinical sample for sequencing which renders the process simple and fast. The use of sputum eliminates the requirement of growing a culture and hence reduces the time delay in diagnosis.
  • Multiple applications – Over and above diagnosis and drug resistance testing, the whole genome sequencing test for Mtb, due to its voluminous data availability, can be used for strain typing, epidemiology studies and disease surveillance.

When do you need to get tested?

A tuberculosis patient can undergo this test in order to diagnose as well as understand drug resistance and susceptibility in the causative microorganism- mycobacterium tuberculosis.

Why MedGenome?

MedGenome SPIT SEQ test is a direct sputum based test that offers multiple applications over conventional methods as follows:

ParameterSmear
Microscopy
Culture
Technique
Nucleic Acid
Amplification
Test (Xpert
MTB/
CBNAAT)
Line Probe
Assay
MedGenome
Whole
Genome
Sequencing of
MTB
Test
application
DiagnosisDiagnosis +
Drug Resistance
Diagnosis +
Drug Resistance
Diagnosis +
Drug Resistance
Diagnosis +
Drug Resistance
Strain
Typing
NoYes, but
confirmation
required
NoNoYes
Specimen
requirement
Direct SputumCultureDirect SputumCultureDirect Sputum
Drugs
for which
resistance
testing can
be done
NAAll (to be tested
individually
for each drug
in a separate
culture)
Rifampicin[3]Rifampicin (RIF)
Isoniazid (INH)
Ofloxacin (OFX)
Amikacin (AMK)[4]
All (done
in a single
sequencing
cycle)
Novel drug
resistance
mutations
coverage
NANA (doesn’t
check for
markers, but
action of the
drug on grown
TB culture)
NoNoYes
Turn around
time
1-2 hours4-8 weeks for
diagnosis and
drug resistance
test
2 hours5 days (once the
culture is grown)[4]
15 working days
Sensitivity55% (among
pulmonary
tuberculosis)[2]
Less for
Conventional
Lowenstein
Jensen- Solid
Media High
for Automated
MGIT- Liquid
Culture
98% in smear
positive cases
70% in smear
negative cases[3]
95.6% for RIF and
INH 74.5% for
OFX and AMK[4]
100%
(compared to
Line Probe
Assay as
reference
standard)
Specificity98% (among
pulmonary
tuberculosis)[2]
NANA90.1% for RIF
and INH[4]
98.6% for OFX
and AMK[4]
98.04%
(compared to
Line Probe
Assay as
reference
standard)

Brochures

SPIT SEQ

FAQ

SPIT SEQ, the direct sputum based whole genome sequencing test for Mtb is validated with 100 samples

Sensitivity100% compared to LPA as reference standard
Specificity98.04% compared to LPA as reference standard

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