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Condition



Test Name

[Combo] Myeloproliferative neoplasm (MPN) reflex panel with BCR-ABL [NGS & RT-PCR]

Inclusive Genes



BCR-ABL RT-PCR if negative JAK2 Sanger sequencing for exons 12 & 14 if negative MPL Sanger sequencing for exon 10 if negativeCALR Fragment analysis of exon 9

Specimen Type

Peripheral blood/bone marrow aspirate/purified genomic DNA

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Transportation Temperature

It is critical that the transportation temperature of peripheral blood/bone marrow aspirate samples are maintained at 2-8⁰C. Alternatively, the samples should be collected in bone marrow tubes. Samples should reach the laboratory within 48 hours of collection.

Volume

Minimum 3ml of peripheral blood; minimum 1ml of bone marrow aspirate; minimum 1 microgram of DNA (concentration of 50-100ng/microliters)

Methodology

Combo test

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