Immunophenotyping Test By Flow Cytometry: Expert Cell Profiling

Flow cytometry is a laboratory technique used to detect, count and analyze he physical and chemical characteristics of individual cells in a fluid suspensions
It identifies and separates cell based on the expression of specific antigens expressed on their surgace/within the cell
The technique relies on the principles of light scattering and fluorescence
It is widely used in the testing of blood, bone marrow and body fluid (cerebrospinal fluid, pleural/pericardial/ascitic fluid)

Why Immunophenotyping by Flowcytometry:

Flow cytometry has been available for several decades and been adapted for use in many areas of clinical testing.

Sub-classification of leukemias and lymphoma
Myelodysplastic Syndrome (MDS) diagnosis
B-MRD (Minimal Residual Disease)- Identification of residual malignant B-Cells (blasts) in B-Cell Acute Lymphoblastic Leukemia after and during the course of treatment
T-MRD (Minimal Residual Disease)- Identification of residual malignant T-Cells (blasts) in T-Cell Acute Lymphoblastic Leukemia after and during the course of treatment
CD34 Stem Cell Enumeration (ISHAGE protocol)
High Sensitivity PNH Assay for diagnosis of paroxysmal nocturnal hemoglobinuria
Multiple Myeloma diagnosis and differentiation of neoplastic myeloma cells from reactive plasma cells
MM-MRD- Identification of residual malignant cells in multiple myeloma
AML-MRD - Identification of residual malignant myeloid blasts in acute myeloid Leukemia after and during the treatment

Why Choose Medgenome Labs For Your Immunophenotyping flow cytometry?

Unique form reporting as per CAP guidelines with cytochemistry examination images (MPO and PAS whenever is required) of blood/ bone marrow films
Scatter plots, analysed images and complete details of immunophenotyping so that the clinicians understands the case in detail
Use of new rare markers as LAIP (Leucocytes Aberrant Immunophenotype), theranostic and prognosis related markers in flow cytometry for future help especially during the course of treatment of patients

MedGenome Offer

Test Code	Test Name	Specimen Type	TAT
MGM997	CD34+ Stem cell Enumeration (CD45, CD34, 7AAD)	Bone marrow/ Peripheral blood in EDTA /Harvest Sample	4 hours
MGM1343	Leukemia/Lymphoma Panel- Flowcytometry	Peripheral blood along with bone marrow aspirate in EDTA	2 working days
MGM412	Acute Leukemia Classifier Panel – Flowcytometry	Peripheral blood along with bone marrow aspirate in EDTA, Peripheral blood can be accepted if blasts/atypical cells are high.	2 working days
MGM413	Acute Leukemia Screen Panel – Flowcytometry	Peripheral blood along with bone marrow aspirate in EDTA. Peripheral blood can be accepted if blasts/atypical cells are high.	2 working days
MGM414	Body fluid Leukemia/Lymphoma screen – Flowcytometry	Body fluid (effusions like CSF, pleural, ascitic, pericardial)	2 working days
MGM415	Chronic Lymphoid Leukemia Panel – Flowcytometry	Peripheral blood along with bone marrow aspirate in EDTA. Peripheral blood can be accepted if blasts/atypical cells are high.	2 working days
MGM417	Myelodysplasia Panel – Flowcytometry	Peripheral blood along with bone marrow aspirate in EDTA	2 working days
MGM416	Lymphoproliferative Disorder Classifier Panel – Flowcytometry	Peripheral blood along with bone marrow aspirate in EDTA. Peripheral blood can be accepted if blasts/atypical cells are high.	2 working days
MGM1230	B MRD	Bone marrow in EDTA	2 working days
MGM1370	PNH by FLAER- High Sensitivity	Peripheral blood in EDTA	2 working days
MGM1374	T- MRD	Bone marrow in EDTA	2 working days
MGM418	Myeloma Panel – Flowcytometry (CD45, CD38, CD19, CD56, CD138, kappa, lambda)	Peripheral blood along with bone marrow aspirate in EDTA	2 working days
–	Multiple Myeloma MRD (MM-MRD)	Bone marrow in EDTA	2 working days

How Is Immunophenotyping Test Is Done?

flowcytometry

Test Detail

Test Code	Test Name	Method	TAT (Working days)	Test Location
MGM2468	Acute Myeloid Leukemia - Minimal Residual Disease (AML-MRD) Inclusive genes/Special Instructions: Provide detailed clinical history along with previous Flow Report	Flowcytometry	2	Bangalore

FAQs

What are the steps involved in flowcytometry tests?

A sample of cells is suspended in a fluid.
Prior to testing and depending on the cells being analyzed, the sample may be treated with special dyes to further define cell sub-types. The dyes (fluorochromes) that are used are attached to monoclonal antibodies that bind to particular cells or key components of cells
The sample containing the cells passes through an instrument called a flow cytometer
In the instrument, the fluid in which the cells are suspended passes through very narrow channels so that the cells are organized in a single file as they pass the detector(s). This is accomplished at a high rate of speed (hundreds to thousands of cells per second.)
The flow cytometer contains one or more lasers and a series of photo detectors that are able to identify certain characteristics unique to various cell types. The single-cell suspension creates unique light-scattering events that occur when each cell passes through the laser light. These initial events are characteristic of the size and shape of the cell, as well as the intensity of the signal that is generated by the specific dyes, thus creating patterns that reflect cell type
The signals from the detectors are amplified and sent to a computer. They are converted to digital read-outs displayed on a computer screen or in a printout
The data are usually displayed as graphs